| Title: | Level of IL-1 and IL-6 in serum and synovial fluid of rheumatoid arthritis patients and their relation to disease activity (Benha Medical Journal, Volume 19 (2002).
Department of rhematology &Rehabilitation and Medical Biochemistry
Benha faculty of Medicine,Zagazig University |
| Authors: | Gamal A.Hamad MD and Fouad EL-Debakey MD |
| Year: | 2002 |
| Keywords: | Not Available |
| Journal: | Not Available |
| Volume: | Not Available |
| Issue: | Not Available |
| Pages: | Not Available |
| Publisher: | Not Available |
| Local/International: | Local |
| Paper Link: | Not Available |
| Full paper | Not Available |
| Supplementary materials | Not Available |
| Abstract: |
Objectives: This experimental study aimed to investigate the possible effect of resveratrol (RSV), an antioxidant agent, during Gentamicin (GEN) treatment as a prophylactic modality against drug induced renal tubular affection. Materials & Methods: The study comprised 30 normal rats divided into 3 equal groups: Control group: did not receive medications, GEN Group: received intraperitoneal (IP) injection of GEN (80 mg/kg/day for 6 days) and RSV+GEN Group; received IP injection of GEN (80 mg/kg/day) and RSV (10 mg/kg/day for 6 days). At the 6th day, blood samples were withdrawn for estimation of serum creatinine, urea and Na and K ions, then all animals were sacrificed and a renal cortex biopsy was obtained for estimation of renal tissue extract activity of glutathione (GSH), glutathione-5- transferase (GST), superoxide dismutase (SOD) and catalase (CAT) and tissue lipid peroxides content expressed as malondialdehyde (MDA). Both kidneys were examined histopathologically and scored for severity of tubular changes. Results: In comparison to control group; GEN caused a significant elevation (p) 0.001) of serum urea and creatinine associated with increased serum Na+ and decreased K+ levels with significantly increased levels of SOD and MDA levels and decreased levels of GSH, GST and CAT in renal tissue extract. On contrary, RSV injection in combination with GEN significantly ameliorated the effects imposed by GEN on the oxidant/antioxidant equilibrium manifested as a non-significant increase of tissue extract levels of MDA associated with non-significant decrease of tissue extract level of GSH and GST in GEN+RSV group compared to control group (P>0.05) but tissue extract activity levels of SOD and CAT were significantly increased (Pi=0.016 & 0.005, respectively) compared to control group. RSV lessened the nephrotoxic effect of Gentamicin in combination group with a significant decrease of serum urea and creatinine levels, (P2==0.005 & 0.008) and a significantly decreased serum Na+ and increased serum K+ compared to levels estimated in GEN group (P2=0.007 & ==0.008, respectively). Light microscopic examination of renal tissue obtained from rats injected with GEN for 6 days, showed degenerative changes with necrosis of proximal tubular cells and contracted glomeruli associated with tubular and glomerular vacuolation. There was tissue edema and intense inflammatory cellular infiltrates. Semi-quantitative analysis of tubular necrosis, tubular vacuolization and parietal cell hyperplasia in animals treated by GEN and GEN+RSV showed a significant reduction of scores of each parameter in GEN+RSV group compared to GEN group with no necrosis reported in 60% and no parietal cell hyperplasia in 30% of animals included in GEN+RSV group, while all GEN treated animals showed tubular necrosis and vacuolation with parietal cell hyperplasia of varied scores. Conclusion: It could be concluded that concomitant administration of resveratrol during Gentamicin therapy in high dose and for long period could ameliorate the structural changes and preserve the oxidant/antioxidant balance in renal tissue with minimization of affection of renal function tests. |
