Presence of Salmonella in samples, 100 each of chicken eggs
and meat, collected from various retail outlets of the Tarai region
of Uttaranchal, was detected by polymerase chain reaction (PCR)
using InvA gene primer, a rapid and sensitive DNA based method.
The results were further validated by established conventional
method of Salmonella isolation and biochemical tests, including
slide agglutination test (poly “O” antiserum specific for
Salmonella). Of 200 samples analyzed, 4 (one meat and 3 egg)
were found to be contaminated with Salmonella enterica var
Typhimurium. Further, the antibiogram of the isolates revealed
sensitivity in the decreasing order to Chloromphenicol, Colistin, |
