Bacterial root endophytes reside in a vast number plant species as a part of their root microbiome. Some of them
have been shown to positively influence in plant growth. Here, six bacterial isolates namely W1, W3, W7, W11,
W12, and W15 were obtained from surface sterilized healthy roots of wheat. This isolates showing positive ability
for IAA production, nitrogen fixation and phosphate solubilization activity. As an indication of favorable
bacterial action, isolateW11 showed the highest functional potentialities in relation to plant growth promoting
activities among the other isolates and increased the total dry weight of root and shoot by 200% and 180%,
respectively. Moreover, the bio-inoculation of wheat root with W11 resulted in significant increase in the N, P
and K concentrations of the shoot after 30 days of inoculation by 82%, 37.5%, and 59%, respectively.
Consequently, the three more efficient isolates namely W11, P31 and P35 were identified as Arthrobacter
arilaitensis, Bacillus anthracis and Achromobacter spanius, respectively, by sequencing of the 16S rRNA gene.
Furthermore, molecular biodiversity of these three isolates were done using eight random primer of RAPD-PCR
and the fragments size ranged from 100-5920 bp. From These results showed that W11 strain proposed as
potential microbial inoculants or biofertilizers for sustainable wheat production in reclamation soil in Egypt
because of its benefit and biosafety. |
