For the first time in the world using the usual technique for isolating plant fungal pathogens, successful growth and sporulation of Uromyces fabae (Pers.) De Bary, the causal of broad bean rust, was achieved axenically on different dilutions (strengths) of a modified MURASHIGE & SKOOG’s (MS-) tissue culture medium. The linear growth of Uromyces fabae was more pronounced on media containing pollen grains only and/or inositol. Regardless media strength, it was found that the MS- medium containing pollen grains at the rate of 10 g/l. was the best for producing uredospores. However, the total number of uredospores was reduced proportionally by increasing dilution of media strength. Few teleutospores were also observed on all tested media. Effect of the tested medium and its components on characters of the resultant uredospores was also included. Under conditions of the present work, uredospores of Uromyces fabae from axenic cultures on MS-medium could not germinate on surfaces of inoculated broad bean leaves and reasonably completely failed to cause any symptoms of rust disease. |
