Senile osteoporosis (SOP) is a degenerative bone disease associated with increasing
susceptibility to fractures and mortality in the elderly. Innate immunity and specifically
the nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin
domain containing 3 (NLRP3) inflammasome, with its subsequent mediators caspase1-
and interlukin-1b (IL-1b),have recently been linked to osteoporosis. Probiotic
lactobacillus acidophilus (L.A) was reported to exert favorable effects on osteoporosis.
The aim of this study was to identify the protective effects of probiotic L.A in aged
osteoporotic rat model and to evaluate the possible underlying mechanisms focusing on
NLRP3 inflammasome and its effectors caspase-1 and interleukin -1b. Thirty-two adult
male albino rats were designated to four equivalent groups. Group I; control, group II;
probiotic L.A, group III; osteoporotic group, and group IV; probiotic LA+ osteoporosis
group. Osteoporotic rats pretreated with L.A in a dose of 109CFU/ml / day for 8 weeks
revealed a significantly lower oxidative stress state, increased bone mineral density
(BMD), enhanced bone histological architecture, lower serum calcium, higher bone
formation markers associated with lower bone resorption marker, lower serum receptor
activator of nuclear factor kappa-Β ligand (RANKL), decreased bone NLRP3
inflammasome as well as caspase-1 expression levels and lower serum IL-
1b.Osteoprotective effects of probiotic L.A in SOP rat model mediated even in part via
its anti-inflammatory effects that was represented by decreased NLRP3 inflammasome
and its subsequent mediators caspase-1 and IL-1b, that resulted in enhancement of bone
formation and reduction of bone resorption. |
