Background: Type 2 diabetes mellitus (T2DM) has injurious impact on liver due to high levels of blood sugar, fats, oxidative
stress, and an increased incidence of inflammation. These factors can consequently lead to various liver disorders.
Aim of the Work: To ascertain impact of T2DM on histological structure of liver molecular and metabolic dysfunction and
the potential protective function of dapagliflozin and moringa.
Materials and Methods: Fifty adult male albino rats were separated into five groups: control group, non-treated diabetic
group, diabetic treated with dapagliflozin group, diabetic treated withMoringa oleifera extract group and diabetic treated
with dapagliflozin plus Moringa oleifera extract group. After 4 weeks, blood samples were taken via cardiac puncture for
biochemical parameters evaluation expression of miRNA 34a, Secretagogin, sirtulin genes also liver samples were processed
for histological and immunohistochemical studies.
Results: Diabetes mellitus induced impairment on liver function with disturbed liver architecture. Administration of
Dapagliflozin and Moringa alone in group III & IV revealed non-significant decrease in mirRNA34a and secretagogin
expression with moderate improvement in biochemical and histological finding. Otherwise, after treatment of diabetic rats
by Dapagliflozin plus Moringa in group V, mirRNA34a and secretagogin expression levels reduced significantly and sirtulin
elevated compared to non- treated diabetic rats. The improvement shown in liver function indicators, oxidative stress markers
and lipid, glucose levels. There is also an improvement in histopathological findings in group V with a significant decrease
in main area distribution of collagen fibers, positive caspase 3 cytoplasmic immunoreactivity and positive PCNA nuclear
immunoreactivity in compared to untreated diabetic group.
Conclusion: Administration of dapagliflozin plus moringa oleifera extract significantly ameliorated the hepatic alteration
induced by diabetes mellitus type 2 in rats. |
