Abstract The objectives of the present study were to investigate strain identification of Echinococcus granulosus
infecting camel and human in Qalyubia, Egypt. Therefore partial sequences were generated after gel purification of
nested PCR amplified products of mitochondrial NADH 1gene of Echinococcus granulosus complex. Sequences
were further examined by sequence analysis and subsequent phylogeny to compare these sequences to those from
known strains of E.granulosus circulating globally and retrieved from GenBank. All isolates are homologous to the
camel strain, E. canadensis (G6) genotype. Nucleotide mutations generate polymorphism at position of 275
nucleotide, where a thymine replaced a cytosine and at the levels of 385 and 386 nucleotides, where two cytosine
substituted a guanine and a thymine respectively. KF815488 Egypt showed typical identity (99.5%) with JN637176
Sudan, HM853659 Iran, AF386533 France and AJ237637 Poland with 0.5% diversion.. Phylogenetic analysis
showed a robust tree clustering all isolates with sequences belonging to the camel genotype (G6) variant with strong
bootstrap values at relevant nodes and the evolutionary distance between groups is very short. There are two
mutations in the sequences of amino acids at the position of 92, where an Alanine is changed to a Valine and at the
position of 129, where a Valine is transformed to a Proline. Our record of a single genotype determined a strain
which could be incriminated for camel and human infectivity and responsible for its persistence in the endemic areas.
Such epidemiological data could guide the application of efficient control strategies of hydatidosis in Egypt.
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