In current study, Yellow perch (P. flavescens) was exposed to common forms of physical stressors and antioxidative effects of dietary incorporated Astragalus membranaceus (AM) and Glycyrrhiza glabra (liquorice) were assessed. To address this, for a four-week five groups of fish (31 ± 1.0 g, average weight) received 1, 2, and 3% (w/w) Glycyrrhiza glabra; and 1% G. glabra- A. membranaceus mixture daily. Control group fed an additive-free basal diet. Immunological, biochemical and histopathological profiles were evaluated; and fish were redistributed to be exposed to heat, cold, hypoxia and capture stressors. The current findings demonstrated that A. membranaceus and G. glabra dietary incorporation remarkably enhanced antioxidative and biochemical parameters. Also, the study showed markedly up-regulation of related genes expression; and revealed better liver histology in supplemented groups over the control. In conclusion, A. membranaceus and G. glabra dietary supplementation markedly enhanced antioxidantive responses throughout the experimental period, indicating the ability of both herbal plants to confer protection against different physical stressors. |
