Amoxicillin/ Clavulanate (AC) combination has become one of the antibiotics most widely prescribed used in the treatment of several bacterial infections, associated with liver injury. The purpose of this study was to examine the modulatory effects of gallic acid (GA) and vitamin C (VTC), both separately and together, on oxidative stress-related liver damage. 64 male albino rats were divided into eight groups at random; negative control; GA group; VTC group; GA +VTC group; AC- treated group; AC + GA- treated group; AC + VTC treated group and AC+ GA + VTC treated group. A twice daily dose of AC (31.83 mg/kg) and a single daily dose of both GA (60 mg/kg) and VTC (200 mg/kg/day) were introduced to rats orally for 7 consecutive days. After sacrificed, blood was collected for biochemical analysis of aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum tumor necrosis factor alpha (TNF-α), caspase-3, RNA of heme oxygenase-1 gene (HMOX-1) and liver sample for lipid peroxidation and histopathological study. In rats given AC, the protein caspase-3 was upregulated together with the serum levels of AST, ALT, and TNF. Following AC delivery, hepatic levels of malondialdehyde (MDA) significantly increased, although reduced glutathione (GSH), glutathione-S-transferase (GST), and HMOX-1 expression levels were decreased. These results were consistent with the histopathology results. Rats receiving GA and/or VTC in addition to AC experienced less liver damage, oxidative stress, apoptosis, and histological changes. We therefore came to the conclusion that GA and VTC had a favourable modulatory impact against AC-induced hepatotoxicity. |
