This study was designed to evaluate the immune response of Equine Herpes Virus-1 (EHV-1) inactivated
vaccine in horses and donkeys in Egypt. The practical part of this study was undertaken in the Veterinary Serum and
Vaccine Research Institute, Abbasia, Cairo. Propagation of EHV-1 local isolate was subjected for further passages
(Passage 4, 6 and 8) on VERO cells. The infectivity titer of the stock virus was 8 log10 TCID50/ ml. Six horses and
six donkeys were used in this study, in addition four Mares and two horses were used for safety test of the prepared
vaccine. Three of horses and three donkeys were vaccinated through intramuscular route by 2 doses of the vaccine
with one Month interval, as well as, another three horses and three donkeys were kept as control. Serum samples
were collected from all vaccinated and control animals every 2 weeks till to the 26th weeks post vaccination (WPV)
which were monitored for EHV-1 antibodies using ELISA, AGPT and VNT. None of the vaccinated horses and
donkeys showed nasal charge, swelling at the site of injection or increase in body temperature post vaccination.
Vaccination of horses and donkeys revealed an increase in the antibodies response against EHV-1 at 2WPV with the
mean ELISA antibodies titer 335.3 and 352 in horses and donkeys respectively. After boostering, antibodies titer is
continuing to increase until reaching to its peak at the 12 WPV with mean antibodies titer 1269.7 and 1153 in horses
and donkeys respectively, then the antibodies titer decreased gradually till to the 26th WPV with a considerable
protective antibody level. Also, AGPT showed precipitin line in collected samples having high antibodies. The
results also revealed an increase in the virus neutralizing antibody titer at 2WPV with the mean antibodies titer 1 and
1.26 in horses and donkeys respectively. After boostering, antibodies titer is continuing to increase until reaching to
its peak at the 12 WPV) with mean antibodies titer 3.1 and 3.16 in horses and donkeys respectively, then the
antibodies titer decreasing gradually till to the 26 WPV with a considerable protective antibody level.
[Mohamed G. Abdelwahab, Hazem M. El Moghazy, Elsayed M. Ibrahim, Faiysal I. Hamouda and Safaa M.A.
Warda. Immune Response Against Inactivated Equine Herpes Virus Vaccine Prepared From Local Isolate In
Horses And Donkeys In Egypt. J Am Sci 2018;14(1):74-83]. ISSN 1545-1003 (print); ISSN 2375-7264 (online).
http://www.jofamericanscience.org. 9. doi:10.7537/marsjas140118.09. |
