Background: Skeletal muscles are susceptible for ischemic reperfusion injury
especially in settings in order to achieve homeostasis in traumatic injury and
vascular surgery. This study aimed at investigating the implication of induction
of diabetes on generation of ischemic reperfusion injury in rat gastrocnemius
muscle. In addition, the possible beneficial effect of metformin and co-enzyme
q10 was investigated.
Methods: About 80 male adult Sprague Dawley rats divided into 10 groups.
Metformin was administrated as continuous oral dose for 28 days. Coenzyme q10
was administrated parenterally 2 and 24 hours before induction of ischemia in
diabetic and non-diabetic animals.
Results: In diabetic ischemic groups, tested drugs either singly or in combination
significantly reduce HB1cA and plasma levels of muscle specific enzyme CPK
and muscle myokin IL6, raised natural antioxidant GSH and reduced oxidative
stress parameters (SOD and MDA), apoptotic (caspase-3) and inflammatory
parameters TNF-α and TGFβ were reduced. Continuous oral metformin for 28
days was more powerful than parenteral short-term coenzyme q10 as regards all
tested parameters except for GSH and caspase-3 both drugs were equi-effective.
Combined drugs have more powerful ameliorating effect than either drug singly
except for HB1cA which was equi-effective with that of metformin. Regarding
non diabetic ischemic groups, metformin was more powerful in reduction of
caspase-3, IL6 and TNF- α while coenzyme q10 was more powerful in elevating
GSH.
Conclusions: Co-enzyme q10 can be used as add on therapy with metformin in
order to decrease the deleterious effects resulted from hind limb ischemia
reperfusion in normal and diabetic rats. |
