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Prof. Hoda Ali Salem El-Garhy :: Publications:

Title:
Lissencephaly-1 gene is required for establishment of axis formation in drosophila melanogaster
Authors: Shereen A.M. Mohamed; Ibrahim I.S. El. Shawaf; Abd El Wahab M. Hassan; Kh. Fahmy and Hoda A.S. El-Garhy
Year: 2016
Keywords: Drosophila, Lissencephaly-1 gene, ectopic expression, anterior-posterior polarity, Segmentation, protein-protein interaction, Axis formation.
Journal: Annals of Agric. Sci., Moshtohor
Volume: 54
Issue: 3
Pages: 621–628
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Hoda Ali Salem El-Garhy_Sheren Abd elhamed Moustafa 621-628.pdf
Supplementary materials Not Available
Abstract:

Lissencephaly-1 gene is known to play many roles during drosophila development, it regulates cyst division, oocyte nucleus migration and control germ stem cell self-renewal hence oocyte formation. In this study we investigated the differential expression of UAS lis-1 RNAi gene construct under the effect of five GAL4 drivers (Nos GAL4, Nub GAL4, Eve 3+7 GAL4, Kni GAL4, Ftz GAL4)on cuticle development. Results showed that Nos and Nub GAL4/UAS lis-1 RNAi have strong effect on abdominal segment development. Meanwhile, Eve 3+7, Kni and Ftz GAL4/UAS lis-1 RNAi showed moderate effect. Proteomic studies (protein- protein interaction) using bicoid (bcd) and even–skipped antibody were used for determining the effect of Nos GAL4/UAS lis-1 RNAi on the expression of maternal and zygotic genes during developmental process. Results showed that Nos GAL4/UAS lis-1 RNAi gene affected slightly on the distribution of the bicoid protein. On the other hand using even-skipped antibody showed alteration in the head length and stripe pattern comparing with control. The overall data showed that UAS lis-1 RNAi gene play an essential role in drosophila axis formation and can be considered as a zygotic effect.

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