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Dr. Howaida Elkholy :: Publications:
Liquid chromatographic method with ultraviolet absorbance detection for measurement of levamisole in chicken tissues, eggs and plasma
|Authors:||El-kholy, HM and Kemppainen, B|
|Journal:||Journal of Chromatography B: Biomedical Sciences and applications|
|Full paper||Not Available|
|Supplementary materials||Not Available|
The development and validation of a high-performance liquid chromatographic and UV detection method was accomplished for quantitative determination of levamisole in chicken tissues, eggs and plasma. The chromatographic separation was achieved on Luna® 5m C18 column using a mobile phase of 0.2% acetic acid in water:methanol (50:50 (v/v)) and Pic B-7 low UV reagent and the pH was adjusted to 7.31 with ammonium hydroxide and UV wavelength was 225 nm. Limits of quantification were 0.025g/g for all tissues and 0.003g/ml for plasma. Limit of detection was 0.001g/g for tissues and plasma. © 2003 Elsevier B.V. All rights reserved.