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Prof. Ibrahim Ibrahim Soliman Elshawaf :: Publications: |
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| Title: | Callus induction and plant regeneration of five Egyptian rice
genotypes as affected by medium constituents |
| Authors: | El-Shawaf I., Hasan A.W., Bekhit M., Hasanein E.S., Salim T. and Bauwe H. |
| Year: | 2011 |
| Keywords: | Oryza sativa, Somatic embryogenesis, Shoot organogenesis, Silver nitrate, Dicamba. |
| Journal: | Arab J. Biotech., Vol. 14, No. (2) |
| Volume: | 14 |
| Issue: | 2 |
| Pages: | 279-290 |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | Not Available |
| Full paper | Ibrahim Ibrahim Soliman Elshawaf_03Callus induction and plant regeneration of five Egyptian rice genotypes as affected by medium constituents.pdf |
| Supplementary materials | Not Available |
| Abstract: |
Callus induction and plant regeneration efficiency of five selected Egyptian rice cultivars, Giza 177, Sakha 101, Sakha 102, Sakha 103, and Sakha 104, were studied using mature embryos as explants. Both plant genotype and medium composition influenced the rate of callus formation. Mature embryo explants of the genotype Sakha 104 grown on N6 medium supplemented with 2 mg l- 1 2,4-D and 8.5 mg l-1 silver nitrate gave the highest callus induction frequency (95%). Among the five genotypes tested, Giza 177, Sakha 104, and Sakha 103 showed the highest frequency of embryogenic callus formation (48.3%, 48%, and 47.3%, respectively), on N6 medium supplemented with 2 mg l-1 dicamba. The highest shoot regeneration efficiency of 75.3 and 70.7%, was observed for Sakha 101 and Sakha 104, respectively, on MS medium supplemented with 1 mg l-1 NAA and 2 mg l-1 kinetin. |
