Background: Glyphosate [N-(phosphonomethyl)glycine] is one of the most widely used 9 organophosphorus herbicides. Allium cepa (AcE), popularly known as onion, has been reported 10 to have an antioxidant properties in both rats and human. 11
The aim of this study is to investigate the histological, and immunohistochemical effects of 12 Glyphosate (GP) as an Organophosphrous compound (OPC) on rat testis and to assess the 13 protective effect of Allium cepa on testis of rats exposed to glyphosate. 14
Materials and methods: This study included 30 adult male Albino rats divided into 15 3groups. Group I (Control group): Each rat received distilled water(0.2 ml/day). Group II: Each 16 rat received glyphosate at a dose of 125 mg /kg, body weight. Group III: Each rat was given 17 Allium cepa (AcE) 1 ml/100 g BW two hours before administration of GP at a dose of 125 mg 18 /kg body weight. All the drugs as well as distilled water were given daily by oral gavages for 19 30days . The sections of testis were stained with Hematoxylin-Eosin (HE) and Masson’s 20 20 Trichrome stains .Also Immunohistochemical study was done to detectBCL2. Morphometric 21 21 study: The mean area percentage of collagen fiber deposition and BCL2 immuno-expression 22 22 was quantified in five images from five non-overlapping fields of each rat . The data were 23 23 collected from the experiment , recorded and analyzed using IBM SPSS Statistics software . 24
RESULTS: The study had demonstrated that glyphosate caused a degeneration of all layers of 25 the germ cells, congestion of the blood vessels, and increased of the collagen fibers in the 26 capsule . Immunohistochemical results showed a decrease in the expression of the 27 antiapoptotic protein(Bcl2) . Allium cepa administration partially ameliorated the degeneration 28 effect of glyphosate on the seminiferous tubules. 29
Conclusion: Allium Cepa protects the testis from the toxic effect of glyphosate 30 |
