The cross-talk between endometrium and embryo is not accessible to the researcher for obvious ethical reasons
that let understand why implantation remains the black box of reproduction. We aimed to detect of the concentrations of IL-1β and TNF-α in endometrial secretion at the time of oocyte retrieval for early prediction of
implantation. One hundred twenty women participated in the study during ICSI cycles. All women participating
in the study included the following criteria; age; 22–36 years, BMI; less than 35 kg/m2
, a husband with oligo- or
oligoasthenospermia. All women received controlled ovarian hyperstimulation and immediately after ovum
pickup, an intrauterine flushing was done. Embryo transfer was done at the blastocyst stage five days after ovum
pick up. Serum pregnancy tests were done for all women. The clinical pregnancy was defined as the appearance
of the gestational sac and positive embryo cardiac activity was confirmed by TVS. The ongoing pregnancy was
detected by abdominal ultrasound at 12 weeks. The participants were divided into two groups: the pregnant
group and the non-pregnant group. Thirty-two and half percent of women got pregnant. There were non-significant differences between the two groups regarding the demographic, clinical and laboratory data except for
the duration of infertility and concentrations of TNF-α and IL-1β. The concentrations of TNF-α and IL-1β were
significantly higher in the pregnant group than the non-pregnant group.Therefore,The use of TNF-α and IL-1β to
predict implantation in IVF is promising especially before embryo transfer.
Clinical trial.gov registration NCT02854514 |
