Liver specimens were taken from seventy fish, Tilapia zillii, living in 3 ecologically different localities in Qalyobya governorate, i.e., unpolluted, agricultural-polluted and industrial-polluted waters of River Nile. Water analysis was carried out to evaluate the
heavy metals in these tested localities. Polymorphism of liver proteins was revealed by SDS-PAGE electrophoresis. Also, DNA damage was detected by counting the fragmentation percent. Results showed that the measured heavy metals were higher in
the agricultural and industrial localities than in control; this was to extent that crossed the permissible concentrations. Fractions of liver proteins showed disappearance and polymorphism in the fish especially that was very close to the outlets of agricultural and industrial drainage. Additionally, both agricultural and industrial water pollution caused an increase in the percentage of DNA fragmentation however the percentage of industrial pollution was higher than in agricultural pollution. The current study aims to revealing the effect of the water pollution, both agricultural and industerial, on the proteinogram and DNA damage in fish inhabiting
these polluted areas of River Nile. The study suggested that there is clear molecular changes in T. zillii; this declares the use of these molecular parameters in diagnosing pollution. |
