Abstract: The immunological genes that may interact with inflammatory postpartum diseases in Italian
buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal
and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was
pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood
was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological
(IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence
differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases
were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p < 0.01), there
were noticeably different probabilities of all major nucleotide changes spreading among buffalo
groups with and without reproductive problems. Buffaloes were significantly more likely to express
the examined genes when they had inflammatory reproductive diseases. The outcomes might
support the significance of these markers’ nucleotide variations and gene expression patterns as
indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo
management policy |
