The immune and antioxidant genetic factors that could converse with mastitis susceptibility
in dromedary camels were looked at in this research. Of 120 female dromedary camels (60
healthy, and 60 with mastitis) were utilised. Each camel's jugular vein was pierced to obtain five
millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants
to obtain whole blood and extract DNA and RNA. The immunological (OTUD3, TLR2,
TLR4, STAB2, MBL2, TRAPPC9, and C4A) and antioxidant (CAT, SOD3, PRDX6, OXSR1, NDUFS6,
SERP2, and ST1P1) genes' nucleotide sequence polymorphisms between healthy and mastitis affected
she-camels were discovered using PCR-DNA sequencing. Fisher's exact test revealed that
camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations
propagating (p < 0.01). Mastitic camels were significantly more likely to express the OTUD3,
TLR2, TLR4, STAB2, MBL2, TRAPPC9, C4A, OXSR1, SERP2, and ST1P1 genes (p < 0.05). However,
CAT, SOD3, PRDX6, and NDUFS6 genes elicited a different pattern. The results may be used to
develop management strategies and support the significance of nucleotide differences and gene
expression patterns in these markers as indicators of the incidence of mastitis. |
