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Dr. Maha Mamdouh Abdel_Gawad Youssef :: Publications: |
|
| Title: | Exploring genetic polymorphisms and transcript
levels of antioxidant and metabolic markers for
prediction and monitoring diarrhea in Holstein
dairy calves |
| Authors: | Ahmed Ateya, Mona Al-Sharif, Salah H. Faraj, Mohamed Abdo, Liana Fericean, Ioan Banatean-Dunea, Salam J. Hammad, Maha Mamdouh, Hayat Fayed, Marawan A. Marawan & Walaa S. Raslan |
| Year: | 2023 |
| Keywords: | Dairy calves; genetic polymorphisms; transcript level; diarrhea |
| Journal: | ACTAActa Agriculturae Scandinavica, Section A — Animal Science |
| Volume: | 53 |
| Issue: | 1904 |
| Pages: | 14-19 |
| Publisher: | taylor |
| Local/International: | International |
| Paper Link: | |
| Full paper | Maha Mamdouh Abdel_Gawad Youssef_Exploring genetic polymorphisms and transcript levels of antioxidant and metabolic markers for prediction and monitoring diarrhea in Holstein dairy ca-1.pdf |
| Supplementary materials | Maha Mamdouh Abdel_Gawad Youssef_Exploring genetic polymorphisms and transcript levels of antioxidant and metabolic markers for prediction and monitoring diarrhea in Holstein dairy ca-1.pdf |
| Abstract: |
The objective of this study was to investigate the genetic polymorphisms and transcript levels of antioxidant and metabolic markers for diarrhea monitoring in Holstein dairy calves. For DNA and RNA extraction, blood samples were taken from 100 female calves (50 with diarrhea and 50 appearing healthy). DNA sequencing and real time PCR for and metabolic genes were performed to identify genetic variants linked to diarrhea susceptibility. Results revealed that there were nucleotide sequence variations in the investigated genes between healthy and diarrheal calves. Keap1, HMOX1, CMPK2, ASPG, FPGT, TNNI3 K, and LPCAT1 were considerably more highly expressed in diarrheic calves than resistant ones. The Nrf2, PRDX2, and PRDX6 genes, however, produced a different pattern. This study provides a practical control strategy to lessen newborn diarrhea in Holstein dairy calves and emphasizes the significance of nucleotide variations along with antioxidant and metabolic gene expression patterns as proxy markers of diarrhea susceptibility/resistance. |
