A simple ISSR-PCR as a routine method of microtuber PVY tested
derived potato plantlets for somaclonal variations is aperquist for pricise
monitoring of quality control during rapid mass micropropagation. and
effective management of microtubers genetic resoures. This study reports
on the use of ISSR-PCR for detection of genetic variations in
micripropagated potato plants. Microtubers PVY tested- derived potato
plantlets were screened using ISSR-DNA markers. Three ISSR primers
were chosen as producing polymorphic DNA fragments differentiating the
investigated plantlets and microtubers in vitro. DNA fingerprint revealed
genetic variations, 40% polymorphisms of therapeutic plantlets,
approximately 50% of the analyzed potato plantlets with 4.5 polymorphic
fragments per primer. While the DNA was isolated from microtubers
produced using jasmonic acid and coumarin after ISSR amplification it
was obvious that microtuber identical fragments profile. The frequency of
somaclonal variations was found to be virus therapeutic and
microtuberization inducers. The somaclonal variations were only detected
in high jasmonic and coumarin concentrations. Although minor
morphological variations were recorded in the microtubers of some clones.
The developed fragments profiles of different micropropagated clones
were typical to that of the donor mother plants.
Key words: DNA fingerprint, ISSR-PCR, microtuber inducers, virus |
