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Prof. Mahmoud Mohamed Hazza :: Publications: |
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| Title: | MOLECULAR IDENTIFICATION OF SOME ISOLATES OF
PSEUDOMONAS AERUGINOSA ISOLATED FROM
PROCESSED FOODS |
| Authors: | Dalia, A.A.Moustafa*; MJVL Hazaa*; Kh.A. El-Doug doug **; Nahed M. Ayaat* and Abeer A. Khattab* *Botany Department, Faculty of Science ,Benha university, Benha, Egypt. **Department of Microbiology, Faculty of Agriculture Ain Shams University Key Words: |
| Year: | 2012 |
| Keywords: | Not Available |
| Journal: | Not Available |
| Volume: | Not Available |
| Issue: | Not Available |
| Pages: | Not Available |
| Publisher: | Not Available |
| Local/International: | Local |
| Paper Link: | Not Available |
| Full paper | Mahmoud Mohamed Hazza_MOLECULAR IDENTIFICATION.pdf |
| Supplementary materials | Not Available |
| Abstract: |
The identification of 16SrRNA structural gene of four Ps .aeruginosa isolates contaminated processd foods was done by PCR and sequencing of amplicons. Genomic DNA isolated from Ps.aeruginosa isolates namely (Ps l,Ps 2,Ps 3,Ps 4)using a lysozyme dodecyl sulfate lysis procedures with high quality and substantly free DNA contamination. The DNA was then used as a template for PCR to amplify the 16srRNA gene via the QLAGEN PCR system by the use of oligo (dt),fGl and rP2 primer sets, partially length of 16srRNA gene could be synthesized . The amplified 16srRNA gene was used as a tempelate using the internal primer combination (fGl and rP2) in PCR to confirm it's specificity to the Ps aeruginosa 16srRNA gene as a PCR product with a size of about 371 bp DNA was amplified of four Ps . aeruginosa isolates. |
