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Prof. Mahmoud Mokhtar Abd El Kader Moustafa :: Publications:

Title:
Silybin content and overexpression of chalcone synthase genes in Silybum marianum L. plants under abiotic elicitation
Authors: Hoda A.S. El-Garhy, Salah Khattabb, Mahmoud M.A. Moustafaa, Rania Abou Ali, Ahmed Z. Abdel Azeiz, Abeer Elhalwagi, Fadia El Sherif
Year: 2016
Keywords: Silybum marianum; Sea water; Gamma rays; LC-MS/MS; Silybin; Chalcone synthase (CHS) genes
Journal: Plant Physiology and Biochemistry
Volume: 108
Issue: Not Available
Pages: 191-202
Publisher: Elsevier
Local/International: International
Paper Link:
Full paper Not Available
Supplementary materials Not Available
Abstract:

Silymarin, a Silybum marianum seed extract containing a mixture of flavonolignans including silybin, is being used as an antihepatotoxic therapy for liver diseases. In this study, the enhancing effect of gamma irradiation on plant growth parameters of S. marianum under salt stress was investigated. The effect of gamma irradiation, either as a single elicitor or coupled with salinity, on chalcone synthase (CHS) gene expression and silybin A + B yield was also evaluated. The silybin A + B content in S. marianum fruits was estimated by liquid chromatography–mass spectrometry (LC-MS/MS). An increase in silybin content was accompanied by up-regulation of the CHS1, CHS2 and CHS3 genes, which are involved in the silybin biosynthetic pathway. The highest silybin A + B production (0.77 g/100 g plant DW) and transcript levels of the three studied genes (100.2-, 91.9-, and 24.3-fold increase, respectively) were obtained with 100GY gamma irradiation and 4000 ppm salty water. The CHS2 and CHS3 genes were partially sequenced and submitted to the NCBI database under the accession numbers KT252908.1 and KT252909.1, respectively. Developing new approaches to stimulate silybin biosynthetic pathways could be a useful tool to potentiate the use of plants as renewable resources of medicinal compounds.

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