SDS-protein and RAPD-PCR markers were used to genetically identify three wheat (Triticum aestivum L.) cultivars (The selected line and the two parents; Sides 6 and Pc 62). The results showed a wide range of variation in SDS-protein banding patterns. The results of SDS-PAGE revealed a total number of 22 bands with molecular weights (MW) ranging from about 16.90 to 96.50 kDa, which were not necessarily present in all the three genotypes. Data revealed all the bands were polymorphic with 100.00 % polymorphism. For RAPD-PCR analysis, thirteen random arbitrary primers were used. OPC8600, OPA15700 and 1268, OPA111292, OPA201383,1238 and 1000, OPC31168 and OPA151901 primers were detected in the Pc 62 parent and the selected line and not found in the Sides 6 parent. Therefore, these six primers are considered as rye genome-specific RAPD markers especially for 5RL segment. The phylogenetic relationships between the three wheat genotypes were determined. The dendrogram tree showed that the selected line and the parental cultivar Sides 6 are very close (similarity of 96.00 %), while Pc 62 cultivar was genetically far from the other genotypes (similarity of 94.00%). In conclusion, the SDS-protein and molecular genetic analysis used in the present study successfully distinguished among different wheat cultivars. |
