Genetic relationships were evaluated among three genotypes of the Ushaar (Calotropis procera Ait (Ait) f.), collected from El Maghara, Shalateen and Sant Kathrin by employing SDS-PAGE for seed protein and peroxidase, polyphenyl oxidase and Alcohol dehydrogenase isozymes analysis. SDS-PAGE generated a total of 12 bands of which nearly 50 % were polymorphic. Two positive bands were detected in the Ushaar genotype from Shalateen at the molecular weights; 230.40 and 175.86 KDa. Moreover, two positive bands were found in the Ushaar genotype from Sant Kathrin at the molecular weights; 168.33 and 148.68 KDa. On the other hand, two negative bands were detected in the Ushaar genotype from Shalateen at the molecular weights; 82.06 and 76.86 KDa, respectively. Identifiable polymorphic bands were detected from peroxidase, polyphenyl oxidase and Alcohol dehydrogenase analysis. However, these polymorphic markers clearly distinguished between the three genotypes from El Maghara, Shalateen and Sant Kathrin regions. The Similarity indices and dendrogram for the genetic distances of the combination between the SDS-PAGE protein and the three isozymes revealed that the highest similarity was 42.90 % between the Ushaar genotypes from Sant Kathrin and Shalateen regions. Meanwhile, the lowest similarity index was between the Ushaar genotypes from El Maghara and Shalateen regions (28.60 %). The dendrogram resulting from the combination of the two techniques separated the three Ushaar genotypes into one cluster (including El Maghara and Sant Kathrin genotypes) with the Shalateen genotype alone.
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