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Prof. Makhlouf Mohamed Mahmoud Bekhit :: Publications:

Title:
Callus induction and plant regeneration of five Egyptian rice genotypes as affected by medium constituents
Authors: El-Shawaf I., Hasan A. M., Bekhit M., Hasanein E. S., Salim T. and Bauwe H.
Year: 2011
Keywords: Oryza sativa, Somatic embryogenesis, Shoot organogenesis, Silver nitrate, Dicamba.
Journal: Arab. Journal of biotechnology. .
Volume: 14
Issue: 2
Pages: 279-290
Publisher: Not Available
Local/International: International
Paper Link:
Full paper Makhlouf Mohamed Mahmoud Bekhit_Tamer Mohamed Shehata Salam_neww-----22.pdf
Supplementary materials Not Available
Abstract:

Callus induction and plant regeneration efficiency of five selected Egyptian rice cultivars, Giza 177, Sakha 101, Sakha 102, Sakha 103, and Sakha 104, were studied using mature embryos as explants. Both plant genotype and medium composition influenced the rate of callus formation. Mature embryo explants of the genotype Sakha 104 grown on N6 medium supplemented with 2 mg l- 1 2,4-D and 8.5 mg l-1 silver nitrate gave the highest callus induction frequency (95%). Among the five genotypes tested, Giza 177, Sakha 104, and Sakha 103 showed the highest frequency of embryogenic callus formation (48.3%, 48%, and 47.3%, respectively), on N6 medium supplemented with 2 mg l-1 dicamba. The highest shoot regeneration efficiency of 75.3 and 70.7%, was observed for Sakha 101 and Sakha 104, respectively, on MS medium supplemented with 1 mg l-1 NAA and 2 mg l-1 kinetin. Keywords: Oryza sativa, Somatic embryogenesis, Shoot organogenesis, Silver nitrate, Dicamba.

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