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Dr. Marwa Mohamed Ali Ibrahim Saleh :: Publications: |
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| Title: | Ameliorative potential of dietary supplements, ZnO‑K,
citrus essential oil, and pumpkin seed oil, on sperm quality
in Nile tilapia: Insights from CASA, DNA integrity,
antioxidant enzymes, and gene expressions |
| Authors: | Marwa M. Ali · Kamal Fathy Elboray · Engy T. Megahed · Hany T. Abu‑Taleb · Alshimaa E. Elsayed · Eman Y. Mohammady · Mona S. Amer · Soliman A. Morsi · Eman M. Abbas · Mohamed S. Hassaan · Hosam Elsaied |
| Year: | 2025 |
| Keywords: | Antioxidant supplements · CASA parameters · DNA integrity · Enzyme bioassay · Gene expression · O. niloticus · Sperm quality |
| Journal: | Fish Physiology and Biochemistry |
| Volume: | 51:114 |
| Issue: | 4 |
| Pages: | 1-15 |
| Publisher: | Springer Netherlands |
| Local/International: | International |
| Paper Link: | |
| Full paper | Marwa Mohamed Ali Ibrahim Saleh_Ameliorative potential of dietary supplements, ZnO‑K,.pdf |
| Supplementary materials | Not Available |
| Abstract: |
Abstract Improving sperm quality is essential for enhancing the reproductive efciency of Oreochromis niloticus. This study evaluated the efect of three dietary antioxidant supplements, kaolinite-doped zinc oxide (ZnO-K), citrus essential oil (CEO), and pumpkin seed oil (PSO), on sperm quality parameters. A suite of integrative assessments, including ComputerAssisted Semen Analysis (CASA) parameters, spermatozoa DNA integrity, antioxidant enzyme assays, and gene expression profling, was applied to validate sperm quality. A total of 192 adult males (mean weight 421.31 g±30.41 SD) were assigned to four dietary groups, each with three replicates. The control group received a basal diet without supplementation. The experimental groups were fed diets supplemented with ZnO-K (0.06 g kg⁻ 1 ), CEO (10 g kg⁻ 1 ), or PSO (15 g kg⁻ 1 ). ZnO-K supplementation signifcantly (P |
