This study aimed to isolate and identify Salmonella spp. From human and chickens by using culture method, serotyping, antimicrobial sensitivity test and detection of virulence genes (invA, avrA, bcfC, stn). A total of 245 samples and swabs including (36 cloacal swabs, 17 intestinal contents, 60 chicken product[ luncheon, nuggets , kofta, pane, 15 of each], 45 chicken meat[ breast, thigh, wing, 15 of each], 20 hand swabs , 27 stool (diarrheic and non- diarrheic ) and 40 serum) from humans for Widal test. The results revealed that 31 samples and swabs were positive to Salmonella, 6 isolates were isolated from broilers cloacal swabs, 7 isolates were isolated from chicken meat (4 in thigh samples and 3 in breast samples and swabs), 2 isolates were isolated from chicken products (one isolate in kofta sample and one isolates in pane swabs), 3 isolates were isolated from intestinal swabs, 4 isolates were isolated from hand swabs from workers in farms and poultry shops and 4 isolates were isolated from stool of diarrheic and non-diarrheic persons while 5 isolates were isolated from serum. Serotyping revealed 9 strains of S. Gallinarum and S. sub spp. salamae mainly in cloacal swabs, while S. Typhimurium, S. Enteritidis, S. Kentucky, S. Tsevie, S. Colindale, S. Papauna and S. Lagos were isolated from chicken meat, its products, hand swab, intestinal swabs and stool. S. Typhi and Paratyphi A, B were isolated from serum of patients by widal test. Nine strains were tested against 6 commercial antibiotics and revealed that all strains were sensitive to levofloxacin and amikacin while were resistant to erythromycin by 100% and ampicillin, tetracycline and cefexime shown viability in sensitivity. Widal test revealed S. Typhi and Paratyphi A, B by a titer of 1:320 for O, H antigens in S. Typhi, Paratyphi A and B. The study detected invA, avrA, bcfC, stn genes in 11 S. Enteritidis and 3 S. Typhimurium that isolated from human and poultry and RAPD PCR revealed relationships in S. Enteritidis that isolated from human and poultry. |
