Background: One-third of the world’s population is infected with Mycobacterium
tuberculosis. Difference in clinical outcome of infection implies that host genetics may be
implicated in such variability. Investigations of Toll-like receptors (TLRs) revealed new
information regarding the immunopathogenesis of tuberculosis. Toll-like receptor 2
(TLR2) mediates crucial immune response against Mycobacterium tuberculosis. There is
argument that Toll-like receptor (TLR10) participate in tuberculosis susceptibility by
acting as a signaling modulator for TLR2. Objectives: The aim of this study was
investigating the relationship between TLR 10 SNP 720A/C (rs11096957) and increase
susceptibility to tuberculosis. Methodology: Eighty patients with radiological,
microbiological and clinical proven active pulmonary tuberculosis (T.B) were included
in this study. (TLR10) polymorphisms and allele distributions were compared between
these 80 patients and 70 healthy control subjects. Peripheral blood samples were taken
from all patients and controls. Genotyping was accomplished by polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP). Results: When we
compare T.B cases with controls, a statistically significant association was observed
between T.B susceptibility and SNP 720A/C (rs11096957) in (TLR10). Allele (A) was
more frequent in tuberculous cases while allele (C) was more common in controls. It was
reported that the AA genotype of (TLR10) SNP rs11096957 was considerably related to
the increased risk of developing pulmonary T.B. Homozygosity (AA) has been associated
with predisposition to disease by comparing cases to controls (P = 0.045; OR = 2.0;
95% C.I. = 1.0- 4.0). A/C heterozygosity was considerably different in tuberculous cases
than in healthy controls with lower risk of developing tuberculosis (P = 0.044; OR =
0.5; 95% C.I. = 0.26 –0.98). Conclusion: TLR10 SNP rs11096957 polymorphism is a
risk factor for tuberculosis infection. |
