This study focuses on Anisakidosis
as a disease caused by a number of Anisakid
larvae including Anisakis simplex,
Pseudoterranova decipiens, Hysterothylacium spp.
and Contracaecum spp., through assessing the
clinical signs and the clinical examination of the
disease in 170 Lizard head (Saurida
undosquamis) and 160 European hakes
(Merluccius merluccius) during the period from
April 2011 to July 2012. In addition,
identification of the recovered larvae
morphologically and using random modified
polymorphic DNA (RAPD-PCR) technique. No
obvious clinical signs were observed in the
examined fish. The post mortem examination
revealed the presence of free or encapsulated
larvae within the body cavity, on the visceral
organs and in the musculature. The seasonal
prevalence of Anisakid larvae was the highest in
spring and summer and its lowest level was in
autumn in both examined fish spp. The highest
intensity of larvae was observed in the organs
and viscera rather than in the musculature.
RAPD technique identified the recovered larvae
using four arbitrary primers. It could be
concluded that Anisakid larvae can be easily
identified using molecular tools by its well
characterized genetic finger print and that in
turn will help in its diagnosis in parallel with the
traditional tools. |
