| Experience |
| Molecular biology such as DNA and RNA extraction, cloning (annealing, ligation, and transformation reactions), plasmid DNA preparations (mini and large scales preps), Sequencing, PCR, genotyping, reverse transcription reaction (RT), digesion reactions, gel extraction and real time PCR). Cell culture techniques such as maintaining and freezing cells, DNA and RNA transfection, cell-based assays such as adhesion, proliferation, migration, invasion, live cell labelling, co cultures and 3D models), immunoflourescence, microscopy (epifluorescence, confocal and electron microscopy), and live-cell imaging microscopy. Mice breading and dissection, Histological preparations, and immunohistochemistry. I also have extensive experiences in western blotting, site directed mutagenesis and have attended workshops in ELISA. Primary cell culture. |
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