The ability of microorganism to adapt the changes in the osmolality of the external milieu is of
fundamental importance for growth and survival, and thus prokaryotic cells have evolved a number of
osmoadaptative mechanisms to cope with elevated osmolality. One hundred bacterial isolates were isolated from soil
from Egypt and screened for salt tolerance. The best four isolates were identified by manual and API 20E system
methods. Identification of bacterial isolates showed that strains were Pseudomonas mallei, Halococcus salifodinae,
Pseudomonas flourecens, Pseudomonas aeruginosa. The primers were designed for screening the bacterial isolates
about the EctC gene from octoine operon. The positive result was with bacterial isolate Pseudomonas mallei. The
length of observed fragment was between 300-400 bp. SDS-PAGE analysis of the four isolates revealed that the
protein profile of the six isolates were different from each other in their banding pattern at different concentrations
of NaCl (0.0 ppm,40.000 ppm and 58.500 ppm) respectively. |
