Diazinon (DZ) is an organophosphorus class II pesticide that is broadly used in agricultural fields and household environments. Ginger (GE) and Atorvastatin (AT) have been shown to have potent anti-oxidative, anti-inflammatory and anti-apoptotic properties in some animal models of toxicity. Here, we investigated the protective properties of GE and AT on DZ-induced renal damage in rats. Total of seven rat groups (7 rats/group) received saline solution (control), ginger (GE), Atorvastatin (AT), Diazinon (DZ), DZ/GE, DZ/AT and DZ/GE/AT through gavage once per day for 30 days respectively. Blood were collected and sera were separated for analysis of kidney failure parameters (Urea and Creatinine). Anti-oxidation parameters were also measured in all the kidneys. Histopathology and IHC of cleaved caspase 3 were also performed on the renal tissues of different groups. Our results showed that GE and AT attenuated DZ-induced renal damage by reduction of malondialdehyde (MDA) and apoptotic (cleaved caspase-3) markers and improving the level of antioxidants and histological picture. Overall, the protective effects of GE and ATR on DZ-induced nephrotoxicity was prominent in DZ intoxicated rats co-treated with one or both compounds and this could be attributed to their potent antioxidative, anti-inflammatory and antiapoptotic properties. |
