Abstract
Background: Type 2 diabetes mellitus (T2DM) is one of the major risk factors for the development of coronary artery disease and subsequent myocardial infarction (MI). ICAM-1 has a vital responsibility in the adhesion of circulating leucocytes to the blood vessel wall and subsequent transendothelial migration to the vascular intima, one of the earliest stepladders in atherogenesis. The object of this study was to investigate the possible role of the K469E polymorphism of the ICAM-1 in genetic susceptibility to MI amongst the patients with type 2 diabetes. Subjects &Methods: The study included 70 patients divided into two groups: Group I included 35 patients suffering from T2DM with MI. Group II included 35 patients with T2DM with no history of coronary artery disease (CAD). In addition, 30 apparently healthy age and gender matched subjects were involved as a control group. For all subjects the followings were done: History taking & clinical examination, ECG, lipid profile, HbA1c, sICAM-1 by ELISA. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used to determine the distribution of allele and genotype frequencies of the 469 E/K polymorphism of the intercellular adhesion molecule-1. Results: Diabetic patients with MI showed a higher statistical significant difference regarding HbA1c compared with diabetic patients without CAD. While, no statistical significant differences were found regarding total cholesterol, LDL-c levels, HDL-c or triglycerides levels between groups I & II. Regarding sICAM1 level, there were high statistical significant differences between diabetic patients with MI (468±40.4 ng/ml) and both diabetic patients without CAD (266.4±44.1 ng/ml) & the healthy controls (150.6±29.7ng/ml p |
