This study presents a method validation for extraction and quantitative analysis of azoxystrobin residues
in green beans and peas using HPLC-UV and the results confirmed by GC–MS. The employed method
involved initial extraction with acetonitrile after the addition of salts (magnesium sulfate and sodium
chloride), followed by a cleanup step by activated neutral carbon. Validation parameters; linearity,matrix
effect, LOQ, specificity, trueness and repeatability precision were attained. The spiking levels for the trueness
and the precision experiments were (0.1, 0.5, 3 mg/kg). For HPLC-UV analysis, mean recoveries ranged
between 83.69% to 91.58% and 81.99% to 107.85% for green beans and peas, respectively. For GC–MS
analysis, mean recoveries ranged from 76.29% to 94.56% and 80.77% to 100.91% for green beans and peas,
respectively. According to these results, the method has been proven to be efficient for extraction and
determination of azoxystrobin residues in green beans and peas.
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