The current study was carried out to study the effect of zinc oxide (ZnO) supported on kaolinite (ZnO-K) on biological parameters, reproduction indices, semen quality, lipid profile and oxidative biomarkers for broodstock of Nile tilapia. Four tested diets were formulated; Diet 1 was control diet without addition. Diet 2, Diet 3 Diet 4 were supplemented with 30, 60 and 90 mg ZnO-K kg−1, respectively. Sixty females with average weight of 135.19 ± 3.91 g and twenty-four males with average weight of 212.51 ± 13.4 g of Nile tilapia (Oreochromis niloticus) were used. All broodstock fish were hand fed experimental diets up to apparent satiation three times daily (9:00, 12.00 and 14.00) for 4 weeks before mating. The highest hepatosomatic index (HSI %) and gonadosomatic index (GSI %) (P < 0.05) of male and female were recorded by fish fed Diet 4. The highest reproductive performance of female includes total absolute relative fecundity and fry survival (P < 0.05) were recorded for fish fed by Diet 4. Regarding semen quality of male, the highest pH, motility and sperm concentration (P < 0.05) were recorded by fish fed diet supplemented with ZnO-K kg−1 especially D4. Serum plasma levels of triglyceride cholesterol and high-density lipoprotein (HDL-C) (P < 0.05) were at higher levels of broodstock male and female fed by D4. The levels of catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD) enzymes were significantly increased (P < 0.05) in the liver of broodstock male and female fed diet supplemented with higher levels of ZnO-K (D4) while, the highest values of alkaline phosphatase (ALP) (P < 0.05) and lower malondialdehyde (MDA) were recorded for fish fed diet without addition (D1). The present result indicated that dietary supplemented with ZnO supported on kaolinite (ZnO-K) at 60 or 90 mg kg−1 has a prominent potential in improving biological parameters and reproduction performance, semen quality, serum lipid metabolism and enzymes activates of Nile tilapia broodstock.
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