A novel and highly selective kinetic spectrophotometric method was developed for the
determination of cyclobenzaprine HCl. This method was based on monitoring the different kinetic characteristics
of the reaction between the studied drug and potassium permanganate as an oxidizing agent in alkaline medium
and the associated change in absorbance by time at λmax 605 nm. The experimental conditions such as the
effects of KMnO4 concentration, amount of NaOH and sequence of addition were optimized. The stoichiometric
ratio and the reaction mechanism between the drug and the oxidant were determined. Different kinetic methods
such as the initial rate and the fixed time methods were utilized under the optimum conditions for construction
the calibration curves for determination cyclobenzaprine HCl. Linear range of 2.0-10 μg mL-1 was established.
The precision of the proposed method was satisfactory where the relative standard deviation (RSD) < 1.89 %
and the error percent (Er %) was in range (-0.33-1.75 %). The proposed method was successfully applied for
determination of cyclobenzaprine HCl in a dosage form. The results were compared with some reported methods. |
