A sensitive and selective spectrofluorimetric method
has been developed for the rapid determination of
europium(III). This method is based on the formation of nonluminous
complex between Eu(III) and a Schiff base reagent
N, N′-bis (salicylidene)-1,2-phenylenediamine (PABD) and
measuring the fluorescence quenching of Eu(III)-PABD complex
at λex/em = 390/577 nm. The fluorescence intensity complex
decreased linearly by increasing the Eu(III) concentration
in the range of 1.0–13.0 μM. The optimum conditions for the
complex formation were determined such as a pH .0 of borate
buffer. The limits of detection (LOD) and quantification
(LOQ) of Eu(III) were determined and found to be 0.217
and 0.653 μM, respectively. The maximum relative standard
deviation of the method for an europium(III) standard of
6.0 μM was 2.07 % (n = 6). The proposed procedures could
be applied successfully for the determination of the investigated
metal ion in some spiked water samples with a good precision
and accuracy compared to official and reported
methods as revealed by t- and F-tests. |
