Pyridoxine is analyzed using square wave voltammetry (SWV) at copper nanoparticles (nano-Cu)
modified poly-crystalline gold electrode (nano-Cu/Au).
Nano-Cu/Au is fabricated by a potential scan electrodeosition technique. Nano-Cu/Au electrode has been characterized morphologically and electrochemically. The analysis of pyridoxine at nano-Cu/Au electrode is achieved utilizing the quenching of copper voltammetric response due
to the complexation with pyridoxine forming an electroinactive complex. Pyridoxine selectively forms complex
with copper ions (modifier), but not with Au (underlying
substrate) as supported by UV/Vis spectrophotometry.
Using SWV the calibration curve for pyridoxine analysis
was obtained in the concentration range of 0.3–2.7 mM
with high correlation coefficient. The proposed method
has been successfully applied for the determination of
pyridoxine in two dosage forms. |
