Herein, the presented manuscript provides for an extensive spectrofluorimetric method for micro determination
of silver ion. This established method based on the use of the three synthesized 2,6-disubstituted pyridine derivatives
(R1, R2 and R3) through exploiting their high fluorescence emission property. A noticeable effect on the
fluorescence emission of the reagents after chelation with Ag (I) was monitored. Its noteworthy that the sensitivity
and stability of this method was increased by using micellar medium. After chelation with Ag(I), the fluorescence
emission of the ligands R1 and R2 were effectively quenched in a regular manner by increasing Ag
(I) concentration. In contrast, an increase of the fluorescence intensity for reagent R3 after addition of Ag
(I) was observed. The solvatochromism for all reagents under investigation was examined in different solvent.
Furthermore, the chelation between Ag(I) and the and designed pyridine reagents was assessed spectrophotometrically.
The optimum conditions for the most stable complexes which give a high signal difference were explored
and well-determined. The linear range for determination of silver ion were determined and found to be
0.18–1.16, 0.06–0.59 and 0.18–1.43 μgmL−1 for R1, R2 and R3, respectively. The statistical analytical parameters
such as LOD, LOQ, SD of slope, SD of intercept and RDSwere calculated. In addition, the developed methodswere
efficaciously applied for determination of Ag(I) in some water samples. These selective complexation methods
found to be in good precision compared to official and reported method as revealed F-test. |
