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Prof. Mohamed EL-Raey Mohamed Metwalli :: Publications: |
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| Title: | EFFECT OF MELATONIN ON BUFFALO BULL SPERM FREEZABILITY, ULTRASTRUCTURE CHANGES AND FERTILIZING POTENTIALS |
| Authors: | MOHAMED EL-RAEY; BADR, M.R.; ASSI, M. M and RAWASH, Z.M. |
| Year: | 2015 |
| Keywords: | Melatonin, semen extender, cryopreservation, HOST, ultrastructure and conception rate. |
| Journal: | Assiut Vet. Med. J. |
| Volume: | 61 |
| Issue: | 144 |
| Pages: | 201-208 |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | |
| Full paper | Mohamed El-Raey Mohamed Metwalli_2-EFFECT OF MELATONIN ON BUFFALO BULL SPERM FREEZABILITY.pdf |
| Supplementary materials | Not Available |
| Abstract: |
Cryopreservation induces sublethal damage to the spermatozoa, which leads to reduce their fertile life. The objective of the present study was to investigate the effect of melatonin on freezability, ultrastructure and fertilizing potentials of the buffalo spermatozoa. Buffalo spermatozoa were cryopresreved with Tris egg yolk extender containing 7% glycerol supplemented with (0.100, 0.250, 0.500, 0.750 and 1 mM melatonin) or Tris-based extender only (control). Cryopresreved spermatozoa were assessed for post-thawing sperm motility, viability and acrosomal integrity, ultrastructure changes and fertilizing potentials. The current results clearly indicated that adding 0.1 mM melatonin to Tris extender significantly improved (P |
