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Prof. Mohamed EL-Raey Mohamed Metwalli :: Publications:

Ameliorative Nexus of Nano Zinc on the Redox State of Chilled and Cryopreserved Barki Ram Semen
Authors: Mohamed RAMADAN 1, Mary G. ABDEL-MALAK2, Gamal A.M. SOSA1, Mohamed EL-RAEY*1
Year: 2023
Keywords: Not Available
Journal: Journal of Advanced Veterinary Research
Volume: 13,
Issue: 5,
Pages: 714-722
Publisher: Not Available
Local/International: International
Paper Link:
Full paper Mohamed El-Raey Mohamed Metwalli_CCF_000360.pdf
Supplementary materials Not Available

The current study aimed to modify rams’ semen redox hemostasis by different concentrations of Nano zinc. Using artificial vagina (15) semen ejaculates were collected, evaluated, and pooled then subjected to processing using different concentrations of nano zinc (10, 50, 100, and 150µg/ml). The concentration of alanine and aspartate transaminases enzyme (ALT-AST), alkaline phosphatase Enzyme (ALP), hydrogen peroxide (H2O2), malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidant capacity (TAC) of chilled and freeze-thawed Barki ram semen were assessed. Nano zinc significantly enhances semen quality in a dose-dependent manner. Where, 150µg/ml of nano zinc potentially reduced the extracellular concentrations of ALT (14.00 ± 1.01 and 28.58 ± 0.58 U/L), AST (39.67 ± 1.54 and54.83 ± 2.33 U/L), ALP (174.90 ± 1.90and 316.80 ± 1.90 U/L), and MDA (5.45 ± 0.02and 6.21 ± 0.01 nmol/ml) after both chilling and freeze-thawing process. Moreover, 150µg/ml treating dose induced an efficient production of SOD (7417±16.67 and 8600±76.38U/ml) after both chilling and freeze-thawing process, respectively. SOD is considered the most important antioxidant enzyme in the sperm cell that scavenges generated free radicles like H2O2. In this respect, 150µg/ml treating dose reported the lowest H2O2 level (0.042 ± 0.002 and0.065 ± 0.005 mM/L), and highest TAC concentration (1.62 ± 0.006and 1.69 ± 0.005 mM/L) after both chilling and freeze-thawing process, respectively. In conclusion, ZnO-NPs in semen extender can modify the sperm redox hemostasis in a dose-dependent manner. It reduces the extracellular transaminases and phosphatases leakage, augmenting SOD activity, and modifies TAC potential. ZnO-NPs supplementation to Barki sperm extender enhances its sperm quality and reduces cryo-injury.

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