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Prof. Mohamed Hussein Kamel Alsayed Mossoua :: Publications:

Title:
Rapid Sero Diagnosis of Pulmonary Tuberculosis through Humoral Immune Response Against 38-kDa and 16-kDa Mycobacterial Antigens
Authors: Rapid Sero Diagnosis of Pulmonary Tuberculosis through Humoral Immune Response Against 38-kDa and 16-kDa Mycobacterial Antigens
Year: 2013
Keywords: Not Available
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Local/International: International
Paper Link:
Full paper Mohamed Hussein Kamel Alsayed Mossoua_367_21715life1004_2743_2748.pdf
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Abstract:

Abstract: Tuberculosis still constitutes a major health problem through the world in spite of major advances in diagnosis, treatment and prevention. Annually, 8 million people become ill with tuberculosis, and 2 million people die from the disease worldwide. Several ELISA tests based on mycobacterial antigens have been used for the rapid diagnosis of Tuberculosis, although demonstration of Mycobacterium tuberculosis in a smear or culture is the most reliable method. The present study aimed to measure the diagnostic value of 16-kDa and 38-kDa mycobacterial antigens in patients who were diagnosed with Tuberculosis by clinical and, or bacteriological findings. This study was carried out in Chest Department of Benha University Hospital on 5 groups which were (40) patients smear positive pulmonary tuberculosis, (20 ) patients smear negative pulmonary tuberculosis, (20) patients inactive pulmonary tuberculosis and control group of (20 ) patients with disease other than tuberculosis. Eg. COPDBroncheactasis, and (20) normal healthy subjects. Medical history, clinical examinations, complete blood picture, ESR, Liver Function Tests, Kidney Function Tests, Fasting Blood Sugar, Tuberculine Skin Test by Montoux Method, sputum for Acid Fast Bacilli by Z.N. stain, pathozyme-TB Complex plus commercial ELISA kit for measuring Immunoglobulin G against 38-kDa and 16-kDa recombinant antigens of all tested groups of TB and control groups. Our findings demonstrated that specificity, sensitivity, positive predictive value, negative predictive value for smear positive group were 97%, 75%, 98.4% and 30% respectively. And for smear negative pulmonary tuberculosis were 95%, 65.2%, 95%, 81% and 80% as regarding specificity, sensitivity, positive predictive value, negative predictive value respectively. Antibodies were detected at above cut-off level in (2) 10% out of (20) subjects with inactive TB. In conclusion, the ELISA Test has a very good specificity and an acceptable sensitivity and positive predictive value for diagnosis and follow-up of Pulmonary Tuberculosis. Recommendation: It is recommended that it could be used in combination with other method to increase the diagnostic accuracy especially for smear negative Tuberculosis cases. [Mohamed H Kamel, Ashraf Talaat, Elsayed Kaoud, Mohamed El-Assal, Ayman M. ELbadawy, Amr M. Elhammady, Ahmed M. Hussein and Azza Mohammed Kamel Abdu Allah. Rapid Sero Diagnosis of Pulmonary Tuberculosis through Humoral Immune Response Against 38-kDa and 16-kDa Mycobacterial Antigens. Life Sci J 2013; 10(4):2743-2748]. (ISSN: 1097-8135).

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