Mimusops Larifolia Leaves butanolic extract contains many phenolic compounds with high antioxidant activity which may have useful health properties including flavonoids, triterpenes, saponins, and amyrins. Oxidative stress is an imbalance between free radical production and the antioxidant defence system which could lead to lipid, protein, and /or DNA alterations. For the present study the strong antioxidant activity of (M. Bu. E) on some blood and serum parameters of male rats exposed to high-fat diet (HFD) -induced oxidative stress were investigated . Sixty male albino rats weighting 150- 200 g. were used. The rats were divided into six equal groups. 1) Control
Normal group: provided only with a constant supply of standard ration. 2) Mimusops laurifolia ( single dose) Group : provided with a constant supply of standard pellet diet, along with administration of M. Bu. E at (75.83 mg/kg b.wt.) orally, once daily for 8 weeks. 3) Mimusops laurifolia ( double dose) Group : provided with a constant supply of standard pellet diet, along with M. Bu. E at ( 151.66 mg/kg b.wt. ), once daily for 8 weeks. 4) HFD Stressed group : fed on high fat diet daily for 8 weeks; for induction of oxidative stress. 5) HFD + M. laurifolia ( Single dose ) Group: fed on high fat diet daily for 8 weeks; along with M. Bu. E at 80 mg/kg b.wt. orally and daily for 8 weeks. 6) HFD + M. laurifolia
( double dose ) Group: fed on high fat diet daily for 8 weeks; along with M. Bu. E at 160 mg/kg b.wt. orally and daily for 8 weeks. Serium and Blood samples were collected from all animals groups, after 4 and 8 weeks from the oncet of experiment. The obtained results revealed that, HFD supplementation exhibited a significant decrease in the levels of SOD, GPx and Nitric oxide whereas it exhibited a significant increase in L-MDA, IL-6, CRP, levels. On contrast, supplementation of M. laurifolia to stressed rats yields a significant reverse estimation of the previously mentioned parameters. The present article revealed the potential pharmacological and phytpchemical effects of Mimusops Larifolia plant as a natural antioxidant and a powerful anti-inflammatory agent against harmful effects of oxidative stress induced by HFD and draw attention for further researches so as to work on untouched pharmacological properties. |
