Assessment [2016-08-25]
Random Amplified Polymorphic DNA (RAPD) markers were employed to assess the genetic diversity and phylogenetic relationships among three Egyptian rabbit breeds (Gabali, Baladi Red and Baladi Black) along with New Zealand White breed as a reference population. A total of 120 animals were used in this study, 30 animals from each breed. Genomic DNA was extracted and PCR amplification was performed and the amplification products were resolved by electrophoresis. A total of 14 RAPD primers of arbitrary sequence were used to amplify pooled genomic DNA from these breeds, and only 10 primers generated reproducible, score able and polymorphic bands. Out of 131 bands scored, 74 bands (56.48%) were recognized as polymorphic and 57 bands (43.52%) as monomorphic. The highest percentage of polymorphic bands was recognized by primers OPB02 (94%) and OPB07 (92%), and the lowest percentage of polymorphic bands was recognized by primers OPA02(16%) and OPF12 (28%). One marker of OPA19 was identified as specific for New Zealand White, three markers detected by OPB02, OPA19 and OPA02 were specific for Baladi Black, four markers detected by OPA19, OPA20, OPB05 and OPB07 in Gabali, and five markers of OPB05, OPB02, OPA19, OPF12 and OPB14 were specific for Baladi Red. The band sharing frequencies (BSF) were found to be the highest between Baladi Red and Baladi Black (0.80± 0.038), followed by Gabali and Baladi Black (0.71±0.079 ), New Zealand White and Baladi Black (0.70±0.096), New Zealand White and Baladi Red (0.69±0.088) and the least BSF was found between New Zealand White and Gabali (0.64±0.081). Overall, there was no significant difference (P>0.05) in BSF values between breeds. The closest genetic distance was found between Baladi Red and Baladi Black (0.87), while the lowest genetic distance was found between New Zealand White and Baladi Black (0.73). download attachment |
Effect of salinity on the genetic variation of olive cultivars grown in Sinai based on ISSR, Isozyme and protein markers. [2016-08-25]
Olive (Olea europaea L.) is one of the most important crops in the Mediterranean area and known by high genetic variation. A comparative account of the polymorphic expression of two antioxidative enzymes (Peroxidase and Super oxide dismutase), one hydrolyzing enzyme (Esterase) and protein electrophoresis was detected from the leaves of four olive cultivars irrigated with different concentrations of saline water. Gel electrophoresis experiments revealed that in most of the cases there were extra numbers of protein bands expressed with relatively low molecular weight in trees irrigated with saline water (2 and 3 bands in Manzanillo, 2 and 1 bands in Picual and 2 bands in case of Dolce cultivar). In all salinity imposed trees, there were sharp increases in band intensity and the number of isoforms of investigated enzyme. Peroxidase increment in trees irrigated with saline water ranged from 2 to 3 polymorphic. Similarly, SOD was shown one band only in all the studied cultivars irrigated or no irrigated with saline water. Increments of Esterase revealed that only one polymorphic band appeared in Eggizi cultivar when irrigated with high concentration of saline water. Genetic variability among four common olive cultivars grown in Sinai, Egypt was assessed 78 fragments of which 29 were polymorphic. The number of polymorphic bands per primer varied from 1 to 6. Genetic similarities were calculated using the Jaccard similarity coefficient. The resulting similarity matrix was subjected to the UPGMA clustering method for dendrogram construction and cultivar differentiation. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies Also, the present results along with those of other researchers show that ISSRs can be used for cultivar differentiation in Olea europaea L. download attachment |
