Vitamin E is a fat soluble vitamin, known with its antioxidant activity. Vit E prevents atherosclerosis and may also help in preventing Alzheimer’s disease. In this study, we tested the protective effects of Vit E and selenium on histology of heart, liver and arteries of Wister rats fed high fat diet. Moreover, the biochemical alterations in parameters of heart and liver function were tested. 80 male Wister rats were divided into 4 groups (20 rats /group), Group I is control, Group II fed high fat diet as control positive. Group III (protective) given 100 mg and 0.1 mg/kg bw/day for 12 weeks Vit E and Selenium respectively with high fat diet. Group IV were injected intraperitoneally Vit E and Selenium 1 g/100 g bw for 12 weeks after feeding high fat diet for 6 months. At the end of experimental design, histological section from liver, heart, blood vessels and so serum changes in metabolites of heart and liver were examined. The tissues of the high fat diet group showed accumulation of small and large fat globules in the hepatocytes, loss of striation in the cardiac muscles and thickening in the wall of the aorta with loss of the elasticity of the elastic membranes in the tunica media. While the addition of vitamin E and selenium to rats together with high fat diet induced protective effects, as Vit E prevents the sever biohazard effects induced by high fat diet alone. The injection of Vitamin E and selenium for 6 months leads to decrease in the degenerative changes occurred in heart, liver and blood vessels with regeneration of these cells. Vit E administered or injected to rats decreased significantly the increase in serum levels of LDL, CPK, GOT induced by feeding high fat diet alone. These results collectively show the benficail effect of Vit E as protective antioxidant vitamin to cardiac and liver biohazards induced by high fat diet and can be used as protective and therapeutical factor in liver, heart and blood vessels diseases. |
