Amylin is a peptide hormone synthesized in b cells of pancreas together with insulin in response to glucose
and nutrients. Amylin controls food intake and body weight but the exact mechanism is still unknown. This
study aimed to examine the effects of amylin injection on hormones, metabolites and cytokines expression
in rats. Wistar rats were injected amylin Intraperitoneally (IP) (10 μg kg-1 twice daily) for 7 days. Plasma
and liver samples were collected for blood measurements and RT-PCR analysis. Amylin treatments induced
significant decrease in body weight and food intake in time dependent manner. Moreover, amylin
significantly increased insulin, leptin and lipase secretion. A decrease in plasma triglycerides, cholesterol
and LPL and increase in HDL levels was reported. There are upregulation in the expression of IL-1b and
TNF-a in amylin treated rats. The results collectively indicate that amylin has anti-obesity like effects
through regulation of proteins and obesity related gene expression. |
