| Title: | BENEFICIAL EFFECTS OF BONE MARROW DERIVED MESENCHYMAL STEM CELLS (BM-MSC) IN FUNCTIONAL RESTORATION OF CHRONIC SPINAL CORD INJURY EXPERIMENTALLY INDUCED IN MALE ALBINO RATS: COMPARISON OF INTRAVENOUS AND INTRALESIONAL ROUTS OF ADMINISTRATION |
| Authors: | Moataz A. Elawady1, Mohammed M. Elmaghrabi1, Nesrine Ebrahim2, Mona A. Elawady3,5Haleem H. ElKasapy, MD-Abde |
| Year: | 2017 |
| Keywords: | spinal cord injury, bone marrow- derived stem cells |
| Journal: | Not Available |
| Volume: | Not Available |
| Issue: | Not Available |
| Pages: | Not Available |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | Not Available |
| Full paper | motaz.youssef_stem cells-final-.pdf |
| Supplementary materials | Not Available |
| Abstract: |
Background:The failure of regeneration after spinal cord injury (SCI) has been attributed to axonal demyelination and neuronal death. The main goal of basic SCI research is to develop new therapeutic interventions which can be applied to prevent or reduce disability Objectives:The aim of the present study was to investigate the effects of rat bone marrow -derived mesenchymal stem cell (BM -MSC) transplantation on the functional restoration of spinal cord injury (SCI) in rats after systemic and intralesional injection. Material and methods:40 adult male albino rats were utilized with an average weight 150-200 grams for each.Animals were divided into four groups: control group, positive control group and 2 experimental groups for intravenous and intralesional injections.The samples was taken from spinal cord around the region of spinal cord injury to study the processes of nerve damage and repair by using Histological study (H&E) staining, immunohistochemical study for detection of nerve regeneration and astrocyte response to the injury. Results:There were obvious differences in the results of the BBB score ,spinal cord immunostaining results after intravenous and intralesional injection in favor of the intralesional route. Conclusions:Bone marrow- derived mesenchymal stem cell transplantation has a promising role in functional restoration of the spinal cord in rats after chronic spinal cord injury especially with the intralesional route of injection. |
