Background: Survivors of myocardial infarction develop scarring followed by 11 ventricular remodeling despite optimum medical care . Stem-cell-based therapy 12 has been given increased attention in terms of its potential contribution to 13 cardiovascular regeneration . However, the therapeutic potential of MSCs is 14 hindered by their low survival rate after transplantation in damaged myocardium 15 The AIM OF THE PRESENT STUDY : Is to find out whether vitamin E can enhance 16 the efficacy of mesenchymal stem cell treatment of isoproterenol-induced 17 myocardial Infarction in rats or not MATERIAL AND METHODS : Fifty Albino rats 18 were divided into 5 equal groups :Group I (Control group): Rats received 1 ml of 19 normal saline SC for 4 weeks. Group II (Isoproterenol group): Rats were given by 20 SC injection 85 mg Isoproterenol / kg.b.w. once daily for two successive days. 21 Group III (Vit E– isoproterenol "ISO" group): Rats were treated with ISO once daily 22 for 2 days and after 1 week, they were received Vit E(100mg/kg b.w./day) orally 23 for 1 week. Group IV (Stem cell group): The rats were treated with ISO for 2 24 days as in group II, and after 1 week from the last dose of ISO, the animals had 25 received MSCs intravenously with 2x1o6 cells/ rat . Group V (stem cell and vit. E 26 group) : The rats were treated with ISO and Vit. E as in group III, and after the last 27 dose of Vit.E, the animals were injected with the MSCs intravenously as in group 28 IV. After rat scarification ,the sections of hearts were stained with Hematoxylin-29 Eosin (HE) and Masson’s Trichrome stains .Also Immunohistochemical study was 30 done to detect caspas-3 and CD105. Morphometric study: The mean area 31 percentage of collagen fiber deposition and Caspase immuno-expression was 32 quantified in five images from five non-overlapping fields of each rat . The data 33 were collected from the experiment , recorded and analyzed using IBM SPSS 34 Statistics software . RESULTS: In ISO group , there were a wide separation of 35
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cardiac muscle fibers with extravasation of blood vessels .In ISP and vit. E group 1 ,there were a moderate separation of cardiac muscle fibers and extravasation of 2 blood vessels . In ISO &stem cell group , there were slight separation of cardiac 3 muscle with minimal extravasation .In ISO ,vit.E and stem cell group ,the cardiac 4 muscle fibers were nearly similar to control group but with minimal extravasation 5 . In this group, there was a minimal amount of collagen fibers when compared 6 with groups II ,III and IV . Also, it showed positive capase -3 immune reaction . As 7 to CD 105 ,this group also showed more positive cytoplasmic reaction in 8 regenerating cardiac muscle . 9
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CONCLUSION: we can conclude that if either Vit.E preparations or stem cells are 11 given alone after myocardial infarction, some improvement of myocardial fibers 12 occurs but when they are given together(VitE. And stem cells), better results are 13 obtained |
