Abstract
Background: Oral mucositis (OM) is mucosal barrier injury. It is commonly caused by 5 Flurouracil (5-FU).
Glucosamine (GlcN) has many beneficial therapeutic effects. Platelets rich plasma (PRP) are a promising line
for curing ofvarious clinical injuries.
Objective: The aim of the study was to evaluate the effect of glucosamine and PRP on experimentally
induced oral mucositis in adult male rats.
Materials and methods: 40 adult male rats divided evenly into four groups. Group I (Control). Group
II (Mucositis): rats injected one time intraperitoneally with 5-FU(150 mg/kg body weight).Group (III)
(Mucositis treated with glucosamine): rats injected intraperitoneally with glucosamine (1000mg/kg body
weight) following induction of oral mucositis for 3weeks. Group IV (Mucositis treated with PRP) rats injected
with PRP(1ml/rat/day) locally into buccal mucosa following induction of oral mucositis for 3weeks. Buccal
mucosa samples prepared and examined by using histological and immunohistochemical techniques.
Results: Group II showed apparent diminish in the thickness of the epithelium with separation of keratin,
hemorrhage and perinuclear vacuolization. There were areas of degeneration, intense subepithelial
mononuclear infiltration and cell apoptosis (highly expressed caspase-3). A significant reduction of Periodic
acid Schiff (PAS) positive reaction and anti-proliferating cell nuclear antigen (PCNA) positive nuclei (P |
